Research enabled by the Zuckerman Institute’s Cellular Imaging Platform

Integrins protect sensory neurons in models of paclitaxel-induced peripheral sensory neuropathy
Collaboration, W1 Spinning Disk Confocal, W1-SoRa Spinning Disk Confocal Cellular Imaging ZI Collaboration, W1 Spinning Disk Confocal, W1-SoRa Spinning Disk Confocal Cellular Imaging ZI

Integrins protect sensory neurons in models of paclitaxel-induced peripheral sensory neuropathy

Grace Ji-eun Shin, Maria Elena Pero, Luke A. Hammond, Anita Burgos, Atul Kumar, Samantha E. Galindo, Tanguy Lucas, Francesca Bartolini, and Wesley B. Grueber (2021) PNAS

In this study, live imaging and superresolution approaches provide evidence that paclitaxel treatment causes cellular changes that are consistent with alterations in endosome-mediated trafficking of integrins. Furthermore, paclitaxel-induced changes in recycling endosomes precede morphological degeneration of nociceptive neuron arbors and could be prevented by integrin overexpression

This was a collaboration between Cellular Imaging and the labs of Wesley Grueber and Francesca Bartolini.

This project used the W1 Spinning Disk Confocal for imaging sensory neuron morphology and the W1-SoRa Spinning Disk Confocal for superresolution imaging of intracellular organelles. Custom image analysis was developed in Cellular Imaging using Fiji and Python.

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